Responses of onion cultivars to sulfur fertilization and their genetic variability under field conditions

Authors

  • Shamil Younis Hassan AL-Hamadany University of Mosul, College of Agriculture and Forestry, Department of Horticulture and Landscape Architecture, Mosul (IQ)
  • Wiam Yahya Rasheed Al-Shakarchy University of Mosul, College of Agriculture and Forestry, Department of Field Crops, Mosul (IQ)
  • Amer Abdullah Hussein Al-Jubouri University of Mosul, College of Agriculture and Forestry, Department of Horticulture and Landscape Architecture, Mosul (IQ)
  • Muhanad A. Ahmad University of Mosul, College of Agriculture and Forestry, Department of Horticulture and Landscape Architecture, Mosul (IQ)
  • Badran S. Agha University of Mosul, College of Agriculture and Forestry, Department of Horticulture and Landscape Architecture, Mosul (IQ)
  • Ahmed AbdelHady Rashedy Cairo University, Faculty of Agriculture, Department of Pomology (EG) https://orcid.org/0000-0002-0297-1594

DOI:

https://doi.org/10.55779/nsb18212935

Keywords:

Allium cepa L., bulb, genetic advance, heritability, yield

Abstract

Sulfur (S) nutrient play a vital role in the formation of aroma, flavor and pungency of onions. This research was conducted under the agro-environmental conditions of College of Agriculture and Forestry, University of Mosul, Nineveh Governorate, Iraq. It was aimed to investigate the impact of four S application levels (0, 45, 90, and 135 kg ha⁻¹) on growth and yield of three onion varieties (Long Red, Round Red, and Round White). For the effect of S level, the highest affected parameters by raising level of S up to 135 kg ha-1 is leaf area, bub weight and  yield which increased over the control by 43.53%, 31.1 and 13.1%, respectively. Regarding  the effect of variety, Long Red variety was significantly higher in leaf area, bulb weight, bulb length  and total yield compared to the other two onion varieties. Regarding the interaction effect (sulfur level x onion variety), the highest S level (135 kg ha-1) improved bulb yield by 20.59, 51.27 and 25.32% for the Long Red, Round Red and Round White, respectively. Genetic variance was significant for all traits except neck diameter and bulb percentage. Broad-sense heritability estimates were particularly high for leaf area and bulb length, indicating a strong genetic contribution to phenotypic expression. Expected genetic advance was high for bulb length, moderate for leaf area, bulb weight, and total yield, and low for the remaining traits. These findings highlight the importance of sulfur fertilization and varietal selection in improving onion productivity and provide valuable insights for future breeding strategies.

 

Metrics

Metrics Loading ...

References

Abdel-Salam M, Abuzaid A, Mahmoud F, Abbas M (2025). Increasing maize productivity in arid sandy soils using combinations of (normal/acidified) biochar and elemental sulfur. Egyptian Journal of Soil Science, 65(1): 339–357. https://doi.org/10.21608/ejss.2024.328587.1887

Abuzaid A, Abdel-Salam M, Abbas M, Khalil F, Abdelhafez A (2025). Effectiveness of biochar and elemental sulfur for sustaining maize production in arid soils. Egyptian Journal of Soil Science, 65(1):163–177. https://doi.org/10.21608/ejss.2024.324620.1875

Ahmed H, Elsherpiny M, Elsaid A (2025). Raising tomato tolerance to soil salinity via foliar application of amino acids and soil addition of sulfur. Egyptian Journal of Soil Science, 65(2): 855–867. https://doi.org/10.21608/ejss.2025.360629.2002

Al-Jassem A (2022). Evaluation of some introduced onion varieties (Allium cepa L.) in terms of growth, morphological characteristics, productivity, and cultivation by seedling method. Syrian Journal of Agricultural Research 9 (4): 103–114.

Al-Kamar MK (1999). Horticultural Plant Breeding. Dar Al-Khaleej Library, Amman, Jordan.

Allard RW (1960). Principles of Plant Breeding. John Wiley and Sons. Inc. New York, USA.

Al-Mufarji OKA (2006). Analysis of combination ability and estimation of hybrid strength and genetic parameters in okra (Abelmoschus esculentus L.). PhD Dissertation, College of Agriculture, University of Baghdad, Iraq.

Al-Mukhtar FA-H (1988). Genetics and breeding of horticultural plants. Ministry of Higher Education and Scientific Research. University of Baghdad, House of Wisdom, Baghdad, Iraq.

Al-Sahouki MM (1990). Yellow Corn: Production and Improvement. Higher Education Press, Baghdad.

Al-Tabbal JA, Al-Fraihat AH (2012). Genetic variation, heritability, phenotypic and genotypic correlation studies for yield and yield components in promising barley genotypes. Journal of Agricultural Science, 4(3): 193–210. https://doi.org/10.5539/jas.v4n3p193

Antonio ES, Santos CA, da Luz LN, Oliveria VR, Candeia JA, Filho JL (2017). Genetic gain of ‘Valenciana’ onion populations developed in the Brazilian semi-arid region. Crop Breeding and Applied Biotechnology, 17: 168–174. https://doi.org/10.1590/1984-70332017v17n2n25

Chattoo MA, Ali A, Kamaluddin (2015). Genetic variability, interrelationship and path analysis for yield and yield-related traits in onion (Allium cepa L.) under temperate condition in Kashmir Valley. Plant Archives, 15(2): 1161–1165.

Chavan S, Hiremath SM, Doggalli G, Patil V, Ajay SM, Vernekar MA, PN VK (2024). Assessment of genetic variability, heritability and genetic advance in white onion (Allium cepa L.) for growth, bulb yield, quality and its component characteristics. Journal of Advances in Biology and Biotechnology, 27(9): 1194–1199. https://doi.org/10.9734/jabb/2024/v27i91389

Corcoles JI, Dominguez A, Moreno MA, Ortega JF, de Juan JA (2015). A non-destructive method for estimating onion leaf area. Irish Journal of Agricultural and Food Research, 54(1): 17–30. https://doi.org/10.1515/ijafr-2015-0002

Dane JH, Topp CG (2020). Methods of soil analysis, Part 4. Physical Methods (Vol 20). John Wiley & Sons. https://doi.org/10.2136/sssabookser5.4

Dargie S, Tariku E, Wslassie M, Gebremedhin G (2016). Response of grain sorghum to split application of nitrogen at Tanqua Abergelle Wereda, North Ethiopia. Journal of Fertilizers and Pesticides, 7(2): 168. https://doi.org/10.4172/2471-2728.1000168

Dobermann A (2007). Nutrient use efficiency—Measurement and management. In: Proceedings of the International Fertilizer Industry Association (IFA) Workshop on Fertilizer Best Management Practices, Brussels, Belgium, 7–9 March 2007, pp. 1–28.

Elbaalawy A, Abouhussien E, Tantawy M, Abd Elhafez E, Nada W (2023). Sulphur compost properties and its amelioration effect on salt affected soil characteristics and productivity. Egyptian Journal of Soil Science, 63(3): 339–354. https://doi.org/10.21608/ejss.2023.214737.1602

Elsherpiny M, Baddour A, EL-Shaboury H (2024). Unveiling the synergistic role of elemental sulfur, potassium supplements and methionine in improving yield and quality of onion. Egyptian Journal of Soil Science, 64(4): 1419–1431. https://doi.org/10.21608/ejss.2024.296109.1788

Farid Y, Ali I, Abdelhafez A, Abbas M (2025). Enhancing wheat productivity in salt-affected soils using traditional and acidified biochars: a sustainable solution. Egyptian Journal of Soil Science, 65(1): 121–134. https://doi.org/10.21608/ejss.2024.325183.1869

Gaurav S, Balaji V, Prasad VM (2017). Studies on multiple correlation between bulb yield, growth and yield attributes in different genotypes of onion (Allium cepa L.) under Allahabad agro-climactic condition. Journal of Pharmacognosy and Phytochemistry, 6(6): 793–798.

González-Morales S, Pérez-Labrada F, García-Enciso EL, Leija-Martínez P, Medrano-Macías J, Dávila-Rangel IE, Benavides-Mendoza A (2017). Selenium and sulfur to produce Allium functional crops. Molecules, 22(4): 558. https://doi.org/10.3390/molecules22040558

Gosai JA, Rathawa SN, Dhakad RK, Jatav A, Verma LR (2018). Evaluation of different varieties of onion (Allium cepa L.) under North Gujarat condition. International Journal Microbiology and Applied Sciences, 7(5): 3775–3780. https://doi.org/10.20546/ijcmas.2018.705.438

Guest A (1966). Flora of Iraq. Ministry of Agric. Republic of Iraq 1: 21.

Hamaiel AF, Abd El-Hady, MAM, Hussien KhR (2020). Impact of sulfur rates and some foliar applications on broccoli. Journal of Plant Production, Mansoura University, 11(9): 835–839. https://doi.org/10.21608/jpp.2020.118031

Hazra P, Som MG (2006). Vegetable Science (1st ed.). Kalyani Publishers.

Hidayatullah, Khan HA, Jillani G (2018). Response of two newly selected onion strains at different plant population. Horticulture International Journal, 2(1): 17–19. https://doi.org/10.15406/hij.2018.02.00019

Howladar S, Semida W, Abd ElMageed T, Kutby A, Howladar M (2025). Sulfur-enriched biochar soil amendment enhances tolerance to drought stress in faba bean (Vicia faba L.) under saline soil conditions. Egyptian Journal of Soil Science, 65(1): 275–290. https://doi.org/10.21608/ejss.2024.324682.1868

Hu W, Wang J, Deng Q, Liang D, Xia H, Lin L, Lv X (2023). Effects of different types of potassium fertilizers on nutrient uptake by grapevine. Horticulturae, 9(4):470. https://doi.org/10.3390/horticulturae9040470

Hulagannavar P, Patil B, Gunnaiah R, Cholin S, Ambresh (2023). Estimates of variability, heritability, genetic advance for yield and its quality traits in onion (Allium cepa L.) genotypes. International Journal of Environment and Climate Change, 13(10): 1758–1770. https://doi.org/10.9734/IJECC/2023/v13i102832

Johnson CH, Robinson HF, Comstock RE (1955). Estimates of genetics and environmental variability in soybean. Agronomy Journal, 47: 314–318. https://doi.org/10.2134/agronj1955.00021962004700070009x

Kempthorne O (1969). An introduction to genetic statistics. Iowa State University Press.

Khallaf S, Moharram A, Moussa L, Hassan S (2025). Bioefficacy of sulfur-oxidizing bacteria and sulfur concentrations in enhancing onion (Allium cepa L.) growth parameters. Egyptian Journal of Soil Science, 65(3): 1587–1603. https://doi.org/10.21608/ejss.2025.404349.2261

Khosa JS, Dhatt AS (2013). Studies on genetic variability and heritability in bulb onion (Allium cepa L.) in North-Western plains of India. Vegetable Science, 40(2): 255–258. https://doi.org/10.24154/jhs.v8i2.314

Lacerda RR de A, Grangeiro LC, Bertino NMF, Gomes VE de V, da Costa JPN, de Almeida AF (2022). Bulb yield and economic viability of onion in response to sulfur fertilization. Revista Brasileira de Engenharia Agrícola e Ambiental, 26(8): 602–609. https://doi.org/10.1590/1807-1929/agriambi.v26n8p602-609

Lancaster JE, Farrant J, Shaw M, Boycott B, Brash D, Armstrong J (2001). Does sulphur supply to the bulb affect storage of onions. Acta Horticulturae, 555: 111–115. https://doi.org/10.17660/ActaHortic.2001.555.13

Lee SY, Kim EG, Park JR, Ryu YH, Moon W, Park GH, Ubaidillah M, Ryu SN, Kim KM (2021). Effect on chemical and physical properties of soil each peat moss, elemental sulfur, and sulfur-oxidizing bacteria. Plants, 10: 1901. https://doi.org/10.3390/plants10091901

Luo D, Tian B, Li J, Zhang W, Bi S, Fu B, Jing Y (2024). Mechanisms underlying the formation of main volatile odor sulfur compounds in foods during thermal processing. Comprehensive Reviews in Food Science and Food Safety, 23(4): e13389. https://doi.org/10.1111/1541-4337.13389

Ma L, Zhao R, Li J, Yang Q, Liu Y (2024). Release characteristics and risk assessment of volatile sulfur compounds in a municipal wastewater treatment plant with odor collection device. Journal of Environmental Management, 354: 120321. https://doi.org/10.1016/j.jenvman.2024.120321

Magray MM, Chattoo MA, Narayan S, Najar GR, Nayeema J, Ahmad T (2017). Effect of sulphur and potassium applications on growth and chemical characteristics of garlic. The Bioscan, 12(1): 471–475.

Mahmood N, Muazzam MA, Ahmad M, Hussain S, Javed W (2021). Phytochemistry of Allium cepa L. (onion): Its nutritional and pharmacological importance. Scientific Inquiry and Review, 5: 41–49. https://doi.org/10.32350/sir/53.04

Manjunath U, Hiremath SM (2022). Genetic variability, heritability and genetic advance in onion (Allium cepa L.) for bulb yield and its component characters. The Pharma Innovation Journal, 11: 2570–2572.

Mather K, Jinks JL (1982). Biometrical Genetics (3rd ed). Chapman and Hall Ltd. London. https://doi.org/10.1007/978-1-4899-3406-2

Matloub AN, Ezz El-Din SM, Karim SA (1989). Vegetable Production (Part Two). Ministry of Higher Education and Scientific Research - University of Mosul.

Muscolo A, Maffia A, Marra F, Battaglia S, Oliva M, Mallamaci C, Russo M (2025). Unlocking the health secrets of onions: investigating the phytochemical power and beneficial properties of different varieties and their parts. Molecules, 30(8): 1758. https://doi.org/10.3390/molecules30081758

Naresh B, Thapa U, Maruthi B, Sruthi D (2025). Genetic analysis of rabi onion (Allium cepa L.) genotypes: Variability, heritability, and genetic advance in the new Gangetic alluvial plains of West Bengal, India. Plant Archives, 25(1): 507–512. https://doi.org/10.51470/PLANTARCHIVES.2025.v25.no.1.076

Ochar K, Kim SH (2023). Conservation and global distribution of onion (Allium cepa L.) germplasm for agricultural sustainability. Plants, 12(18): 3294. https://doi.org/10.3390/plants12183294

Pii Y, Cesco S, Mimmo T (2015). Shoot ionome to predict the synergism and antagonism between nutrients as affected by substrate and physiological status. Plant Physiology and Biochemistry, 94: 48-56. https://doi.org/10.1016/j.plaphy.2015.05.002

Przygocka-Cyna K, Barłóg P, Grzebisz W, Spiżewski T (2020). Onion (Allium cepa L.) yield and growth dynamics response to in-season patterns of nitrogen and sulfur uptake. Agronomy, 10(8): 1146. https://doi.org/10.3390/agronomy10081146

Pujar UU, Jagadeesha RC, Gangadharappa PM, Chavan ML, Shankarappa S, Jayappa J (2019). Assessment of genetic variability in onion (Allium cepa L.) genotypes. Indian Journal of Pure & Applied Biosciences, 7(6): 152–156. http://dx.doi.org/10.18782/2582-2845.7889

Pushpendra S, Sony AK, Diwaker P, Meena AR, Sharma D (2017). Genetic variability assessment in onion (Allium cepa L.) genotypes. International Journal of Chemical Studies, 5(5): 145–149.

Rahul C, Singh S, Kumar A, Taak Y, Khar A (2023). Genetic diversity of morphological, biochemical and mineral traits in Indian onion (Allium cepa) genotypes. The Indian Journal of Agricultural Sciences, 94(6):140320. https://doi.org/10.56093/ijas.v94i6.140320

Rajshree G, Kimneihchong G, Jitendra T, Kiran N (2025). Genetic variability and character association analysis in white onion (Allium cepa L.) genotypes. International Journal of Advanced Biochemistry Research, 9(9S): 921–926. https://doi.org/10.33545/26174693.2025.v9.i9Sl.5618

Rajya RL (2015). Studies on genetic variability, correlation and path analysis of yield and yield components in onion. Journal of Horticultural Science, 10(2): 237–241. https://doi.org/10.24154/jhs.v10i2.137

Ren F, Zhou S (2021). Phenolic components and health beneficial properties of onions. Agriculture, 11(9): 872. https://doi.org/10.3390/agriculture11090872

Rietra RPJJ, Heinen M, Dimkpa CO, Bindraban PS (2017). Effects of nutrient antagonism and synergism on yield and fertilizer use efficiency. Communications in Soil Science and Plant Analysis, 48(16): 1895–1920. https://doi.org/10.1080/00103624.2017.1407429

Sabina I, Khar A, Singh S, Tomar BS (2023). Variability, heritability and trait association studies for bulb and antioxidant traits in onion (Allium cepa) varieties. The Indian Journal of Agricultural Sciences, 89(3): 87588. https://doi.org/10.56093/ijas.v89i3.87588

Samborska IA, Kalaji HM, Sieczko L, Borucki W, Mazur R, Kouzmanova M, Goltsev V (2019). Can just one-second measurement of chlorophyll a fluorescence be used to predict sulphur deficiency in radish (Raphanus sativus L. sativus) plants? Current Plant Biology, 19: 100096. https://doi.org/10.1016/j.cpb.2018.12.002

Sami R, Elhakem A, Alharbi M, Benajiba N, Almatrafi M, Helal M (2021). Nutritional values of onion bulbs with some essential structural parameters for packaging process. Applied Sciences, 11(5): 2317. https://doi.org/10.3390/app11052317

Santra P, Manna D, Sarkar HK, Maity TK (2017). Genetic variability, heritability and genetic advance in Kharif onion (Allium cepa L.). Journal of Crop and Weed, 13(1): 103–106.

Sarker U, Islam MT, Rabbani MG, Oba S (2015). Variability, heritability and genetic association in vegetable amaranth (Amaranthus tricolor L.). Spanish Journal of Agricultural Research, 13(2): e0702. https://doi.org/10.5424/sjar/2015132-6843

SAS (2017). SAS software (Version 9.4). SAS Institute Inc.

Segundo VCV, Innecco R, Freitas JA, Lima EN, Nogueira APO, Luz JMQ (2022). Genetic parameters and diversity, and correlations in onion strains. Revista Caatinga, 35(2): 352–362. https://doi.org/10.1590/1983-21252022v35n211rc

Shah SH, Islam S, Mohammad F (2022). Sulphur as a dynamic mineral element for plants: a review. Journal of Soil Science and Plant Nutrition, 22(2): 2118–2143. https://doi.org/10.1007/s42729-022-00798-9

Sharma PK, Singh A, Duhan DS, Kishor N, Barar NS (2017). Genetic variability, heritability and genetic advance in onion (Allium cepa var. Cepa L.). International Journal of Pure and Applied Bioscience, 5(6):740-743. https://doi.org/10.18782/2320-7051.6044

Sparks DL, Page AL, Helmke PA, Loeppert RH (2020). Methods of soil analysis, part 3: Chemical methods, 14, John Wiley & Sons. https://doi.org/10.2136/sssabookser5.3

Steel RGD, Torrie JH (1980). Principles and procedures of statistics: A biometrical approach (2nd ed.). McGraw-Hill Book Company.

Surbhi S, Ameta KD, Chhipa BG, Dubey RB, Saharan V (2024). Genetic variability analysis in onion (Allium cepa L.) genotypes. Indian Journal of Arid Horticulture, 6(1): 16401. https://doi.org/10.48165/ijah.2024.6.1.8

Tabak M, Lisowska A, Filipek-Mazur B, Antonkiewicz J (2020). The effect of amending soil with waste elemental sulfur on the availability of selected macroelements and heavy metals. Processes, 8: 1245. https://doi.org/10.3390/pr8101245

Tripathy P, Sahoo BB, Priya DA, Das SK, Dash DK (2013). Effect of sources and levels of sulphur on growth, yield and bulb quality in onion (Allium cepa L.). International Journal of Bioresources and Stress Management, 4(4): 641–644.

Tripathy T, Sahoo BB (2018). Genetic variability, heritability and genetic advancement in Rabi onion (Allium cepa L.). Journal of Allium Research, 1(1): 37–40.

Welsh JR (1981). Fundamentals of Plant Genetics and Breeding. John Wiley & Sons, Inc. New York, U.S.A.

Yebalework WA, Robi ND, Hora DA, Chala EE, Abebe YA (2024). Nitrogen and sulfur fertilizer application on subsequent storage potential and quality of onion (Allium cepa) bulb in the Central Rift Valley of Ethiopia. Agricultural Research, 13(2): 300–308. https://doi.org/10.1007/s40003-024-00703-z

Zenda T, Liu S, Dong A, Duan H (2021). Revisiting sulphur, the once neglected nutrient: its roles in plant growth, metabolism, stress tolerance and crop production. Agriculture, 11(7): 626. https://doi.org/10.3390/agriculture11070626

Downloads

Published

2026-06-27

How to Cite

AL-Hamadany, S. Y. H., Al-Shakarchy, W. Y. R., Al-Jubouri, A. A. H., Ahmad, M. A., Agha, B. S., & Rashedy, A. A. (2026). Responses of onion cultivars to sulfur fertilization and their genetic variability under field conditions. Notulae Scientia Biologicae, 18(2), 12935. https://doi.org/10.55779/nsb18212935

Issue

Section

Research Articles
CITATION
DOI: 10.55779/nsb18212935