Preliminary safety evaluation and probiotic potential of a tempeh-derived lactic acid bacteria consortium in Wistar rats

Siti Nur Azizah; Rosida Rosida; Rizka Yolanda Febiaocti

doi:10.55779/nsb18212879

Authors

Siti Nur Azizah Politeknik Kesehatan Jember, Pharmacy Vocational Program, 68125 Jember, East Java (ID) https://orcid.org/0009-0004-0882-5880
Rosida Rosida Politeknik Kesehatan Jember, Pharmacy Vocational Program, 68125 Jember, East Java (ID) https://orcid.org/0000-0003-1873-1525
Rizka Yolanda Febiaocti Politeknik Kesehatan Jember, Medical Laboratory Technology Vocational Program, 68125 Jember, East Java (ID) https://orcid.org/0009-0000-7830-6438

DOI:

https://doi.org/10.55779/nsb18212879

Keywords:

consortium, LAB, probiotic, tempeh, Wistar

Abstract

The lactic acid bacteria (LAB) consortium isolated from tempeh produced in Jember, East Java, Indonesia, represents a promising probiotic candidate. This study aimed to evaluate the preliminary safety and probiotic potential of the LAB consortium administered at high doses for 28 days in Wistar rats. This study employed a true experimental design consisting of four groups: healthy (P1), milk (P2), LAB consortium at a dose of 10¹¹ CFU mL⁻¹ rat⁻¹ day⁻¹ (P3), and LAB consortium at a dose of 2 ×10¹¹ CFU mL⁻¹ rat⁻¹ day⁻¹ (P4). Throughout the experiment, feed intake, body weight, and fecal consistency were monitored daily and subsequently analyzed. On day 29, blood samples were collected for hematological and serum biochemical analyses, whereas ileum, colon, and fecal samples were collected for histological and microbiological examinations. The results demonstrated that high-dose LAB consortium treatment (P4) did not produce any effects on general health status, organ weights index, hematological, clinical chemistry, or ileum histoanatomy, all of which were comparable to those observed in the healthy group (P1). Administration of the LAB consortium (P4) also contributed to an increase in the LAB population in the colon, reaching 10⁸ CFU mL⁻¹, which was higher than that observed in the other groups. The fecal bacterial profile revealed that Shigella was undetectable in group P4. The population of Escherichia coli remained relatively stable across all groups, while Salmonella was absent in all. Therefore, administration of the LAB consortium provides preliminary evidence of safety and indicates its potential for development as a probiotic candidate.

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