Osmoprotective mechanisms of compatible solutes in Enterococcus faecium LBO1 isolated from traditional Algerian Lben
DOI:
https://doi.org/10.55779/nsb17412750Keywords:
compatible solutes uptake, Enterococcus faecium, intracellular metabolites analysis, membrane lipid remodelling, osmoadaptation, osmotic stressAbstract
This study investigated the osmoadaptation strategies of Enterococcus faecium strains isolated from traditional Algerian dairy products, focusing on their potential as starter cultures for food industry. In this context, eleven isolates were confirmed as E. faecium (16S rRNA, 99.8%). The strain LBO1 showed the highest salt tolerance, growing at 9.5% (w/v) NaCl with a minimum inhibitory concentration of 10% (w/v). Its response to hyperosmotic stress was evaluated with five compatible solutes: proline, sodium glutamate, sorbitol, mannitol, and maltose. Our findings indicate proline and sodium glutamate (0.12% w/v) significantly enhanced growth and viability. GC–MS profiling revealed intracellular accumulation of trehalose and sorbitol, while lipid analysis showed a 2.3-fold increase in unsaturated fatty acids and the appearance of cyclic fatty acids. Increased cell surface hydrophobicity indicated membrane remodelling under stress. Principal component analysis highlighted the combined contribution of solute uptake and membrane lipid adjustments to salt tolerance. These findings demonstrate that E. faecium LBO1 adapts to osmotic stress through compatible solutes and membrane reorganisation, supporting its potential as a safe and effective culture for saline dairy fermentations.
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