Effect of the use of biostimulants on the yield and quality of tomato (Solanum lycopersicum L.) grown in a greenhouse
DOI:
https://doi.org/10.55779/nsb17412745Keywords:
biostimulant, greenhouse, quality, tomato, yieldAbstract
Tomato (Solanum lycopersicum L.) is among the most important horticultural crops globally, and interest in improving both yield and quality through sustainable strategies is increasing. This study assessed the effects of a biostimulant based on humic acids and microorganisms on the yield and organoleptic quality of two saladette tomato varieties (Macizo and Moctezuma) under greenhouse conditions. A randomized complete block design with four replicates compared control treatments (CT) without biostimulant to biostimulant treatments (BT). Biostimulant application significantly increased yield in both varieties (p<0.05), with Macizo rising from 27 to 37.5 kg/plant (39%) and Moctezuma from 28.5 to 42 kg/plant (47%). Quality improvements included greater equatorial diameter in Moctezuma and increased firmness in Macizo (p<0.05). After 14 days at 19 °C, BT fruits lost significantly less weight than CT fruits. Flavonoid content increased in BT (Macizo: 4.46 vs 1.48 mg QE/100 g; Moctezuma: 3.36 vs 1.25 mg QE/100 g), while antioxidant activity and total phenolics decreased (p<0.05). No differences were found in color, soluble solids, or titratable acidity (p>0.05). These results indicate that biostimulants can enhance tomato yield and some quality traits, but their influence on secondary metabolites may differ depending on variety and cultivation conditions.
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References
Abdellatif IMY, Abdel-Ati YY, Abdel-Mageed YT, Hassan MAMM (2017). Effect of humic acid on growth and productivity of tomato plants under heat stress. Journal of Horticultural Research 25(2):59-66. https://doi.org/10.1515/johr-2017-0022
Aghaeifard F, Babalar M, Fallahi E, Ahmadi A (2016). Influence of humic acid and salicylic acid on yield, fruit quality, and leaf mineral elements of strawberry (Fragaria × ananassa Duch.) cv. Camarosa. Journal of Plant Nutrition 39(13):1821-1829. https://doi.org/10.1080/01904167.2015.1088023
Al-Dairi M, Pathare PB, Al-Yahyai R (2021). Effect of postharvest transport and storage on color and firmness quality of tomato. Horticulturae 7(7):163. https://doi.org/10.3390/horticulturae7070163
Ali N, Farrell A, Ramsubhag A, Jayaraman J (2016). The effect of Ascophyllum nodosum extract on the growth, yield and fruit quality of tomato under tropical conditions. Journal of Applied Phycology 28(2):1353-1362. https://doi.org/10.1007/s10811-015-0608-3
Almuslimawi AAA, Kuchár B, Navas SEA, Turóczi G, Posta K (2024). The effect of combined application of biocontrol microorganisms and arbuscular mycorrhizal fungi on plant growth and yield of tomato (Solanum lycopersicum L.). Agriculture 14(5):768. https://doi.org/10.3390/agriculture14050768
Al-Saif AM, Ahmed MEM, Taha MA, Sharma A, El-Sheshtawy ANA, Abouelsaad IA, El-Serafy RS, Mahdy RM (2024). Preharvest applications improve the postharvest storage and quality of tomato fruits by enhancing the nutritional value and antioxidant system. Horticulturae 10(12):1248. https://doi.org/10.3390/horticulturae10121248
Awad MA, Asiry KA, Elsayed MI, Abo-Elyousr KAM, Alqarni NS, Al-Qurashi AD, Mousa MA (2024). Effect of black soldier fly larvae frass as organic fertilizer on postharvest quality and shelf life of open-field-grown tomato (Solanum lycopersicon L.). HortScience 59(11):1603-1608. https://doi.org/10.21273/hortsci18086-24
Batt PJ (2006). Fulfilling customer needs in agribusiness supply chains. Acta Horticulturae 699:83-89. https://doi.org/10.17660/ActaHortic.2006.699.8
Bisbis MB, Gruda N, Blanke M (2018). Potential impacts of climate change on vegetable production and product quality—A review. Journal of Cleaner Production 170:1602-1620. https://doi.org/10.1016/j.jclepro.2017.09.224
Brand-Williams W, Cuvelier ME, Berset CLWT (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food science and Technology 28(1):25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
Bulgari R, Franzoni G, Ferrante A (2019). Biostimulants application in horticultural crops under abiotic stress conditions. Agronomy 9(6):306. https://doi.org/10.3390/agronomy9060306
Calvo P, Nelson L, Kloepper JW (2014). Agricultural uses of plant biostimulants. Plant and soil 383(1):3-41. https://doi.org/10.1007/s11104-014-2131-8
Canellas LP, Olivares FL, Aguiar NO, Jones DL, Nebbioso A, Mazzei P, Piccolo A (2015). Humic and fulvic acids as biostimulants in horticulture. Scientia Horticulturae 196:15-27. https://doi.org/10.1016/j.scienta.2015.09.013
Carmody N, Goñi O, Łangowski Ł, O’Connell S (2020). Ascophyllum nodosum extract biostimulant processing and its impact on enhancing heat stress tolerance during tomato fruit set. Frontiers in Plant Science 11:807. https://doi.org/10.3389/fpls.2020.00807
Choudhary D, Rawat M, Mashkey VK, Sharma V, Kundu P (2025). Estimation of seaweed extract and micronutrient potential to improve net returns by enhancing yield characters in tomato using correlation analysis. Journal of Applied Biology & Biotechnology 13(1):243-249. https://doi.org/10.7324/JABB.2024.199958
Cirillo V, Romano I, Woo SL, Di Stasio E, Lombardi N, Comite E, Pepe O, Ventorino V, Maggio A (2023). Inoculation with a microbial consortium increases soil microbial diversity and improves agronomic traits of tomato under water and nitrogen deficiency. Frontiers in Plant Science 14:304627. https://doi.org/10.3389/fpls.2023.1304627
Colla G, Cardarelli M, Bonini P, Rouphael Y (2017). Foliar applications of protein hydrolysate, plant and seaweed extracts increase yield but differentially modulate fruit quality of greenhouse tomato. HortScience 52(9):1214-1220. https://doi.org/10.21273/hortsci12200-17
Dai J, Mumper RJ (2010). Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules 15(10):7313-7352. https://doi.org/10.3390/molecules15107313
Dasgan HY, Aksu KS, Zikaria K, Gruda NS (2024). Biostimulants enhance the nutritional quality of soilless greenhouse tomatoes. Plants 13(11):2587. https://doi.org/10.3390/plants13182587
Dávila-Aviña JE, Villa-Rodríguez JA, Villegas-Ochoa MA, Tortoledo-Ortiz O, Olivas GI, Ayala-Zavala JF, González-Aguilar GA (2014). Effect of edible coatings on bioactive compounds and antioxidant capacity of tomatoes at different maturity stages. Journal of Food Science and Technology 51(10):2706-2712. https://doi.org/10.1007/s13197-012-0771-3
Di Mola I, Ottaiano L, Cozzolino E, Marra R, Vitale S, Pironti A, Fiorentino N, Mori M (2023). Yield and quality of processing tomato as improved by biostimulants based on Trichoderma sp. and Ascophyllum nodosum and biodegradable mulching films. Agronomy 13(3):901. https://doi.org/10.3390/agronomy13030901
Dias JS (2012). Nutritional quality and health benefits of vegetables: A review. Food and Nutrition Sciences 3(10):1354-1374. https://doi.org/10.4236/fns.2012.310179
Do QD, Angkawijaya AE, Tran-Nguyen PL, Huynh L., Soetaredjo FE, Ismadji S, Ju YH (2014). Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis 22(3):296-302. https://doi.org/10.1016/j.jfda.2013.11.001
Dobbs LB, Medici LO, Peres LEP, Pino‐Nunes LE, Rumjanek VM, Façanha AR, Canellas LP (2007). Changes in root development of Arabidopsis promoted by organic matter from oxisols. Annals of Applied Biology 151(2):199-211. https://doi.org/10.1111/j.1744-7348.2007.00166.x
Du Jardin P (2015). Plant biostimulants: Definition, concept, main categories and regulation. Scientia Horticulturae 196:3-14. https://doi.org/10.1016/j.scienta.2015.09.021
Elkeleny SS, Mahmoud AK, Abou-Elnasr HS (2024). Effect of some postharvest treatments on removal of emamectin benzoate and quality of cherry tomato fruits during cold storage. Horticulture Research Journal 2(3):1-21. https://doi.org/10.21608/hrj.2024.378550
FAO, FAOSTAT: Estadísticas de producción agrícola (2023). Organización de las Naciones Unidas para la Alimentación y la Agricultura. https://www.fao.org/faostat/es/
Farneti B (2014). Tomato quality: From the field to the consumer – interactions between genotype, cultivation and postharvest conditions. PhD Thesis. Wageningen University. https://hdl.handle.net/11585/389383
Filho JFDCL, Thomason WE, Evanylo GK, Zhang X, Strickland MS, Chim BK, Diatta AA (2020). The synergistic effects of humic substances and biofertilizers on plant development and microbial activity: A review. International Journal of Plant & Soil Science 32(7):56-75. https://doi.org/10.9734/IJPSS/2020/v32i730306
Galambos N, Compant S, Moretto M, Sicher C, Puopolo G, Wäckers F, Sessitsch A, Pertot I, Perazzolli M (2020). Humic acid enhances the growth of tomato promoted by endophytic bacterial strains through activation of hormone-, growth-, and transcription-related processes. Frontiers in Plant Science 11:582267. https://doi.org/10.3389/fpls.2020.582267
Ganugi P, Fiorini A, Tabaglio V, Capra F, Zengin G, Bonini P, Caffi T, Puglisi E, Trevisan M, Lucini L (2023). The functional profile and antioxidant capacity of tomato fruits are modulated by the interaction between microbial biostimulants, soil properties, and soil nitrogen status. Antioxidants 12(2):520. https://doi.org/10.3390/antiox12020520
Golin MF, Giannini V, Bagarello M, Cartagena WCV, Giordano M, Maucieri C (2024). Processing tomato and potato response to biostimulant application in open field: an overview. Agronomy 14(11):2699. https://doi.org/10.3390/agronomy14112699
González-Pérez L, Tejera E (2024). Hydroxycinnamic acid oligomers-based biostimulant Nurspray® enhances tomato yield, fruit quality, and reduces blossom-end rot. Agronomy 14(10):2317. https://doi.org/10.3390/agronomy14102317
Gorni PH, de Lima GR, Pereira LMO, Spera KD, Lapaz ADM, Pacheco AC (2022). Increasing plant performance, fruit production and nutritional value of tomato through foliar applied rutin. Scientia Horticulturae 294:110755. https://doi.org/10.1016/j.scienta.2021.110755
Haider STA, Anjum MA, Shah MN, Hassan AU, Parveen M, Danish S, Alharbi SA, Alfarraj S (2024). Deciphering the effects of different calcium sources on the plant growth, yield, quality, and postharvest quality parameters of ‘tomato’. Horticulturae 10(9): 1003. https://doi.org/10.3390/horticulturae10091003
Hernández V, Hellín P, Fenoll J, Cava J, Garrido I, Molina MV, Flores P (2016). The use of biostimulants can mitigate the effect of high temperature on productivity and quality of tomato. Acta Horticulturae 1194:85-90. https://doi.org/10.17660/ActaHortic.2018.1194.14
Ikuyinminu E, Goñi O, O’Connell S (2022). Enhancing irrigation salinity stress tolerance and increasing yield in tomato using a precision engineered protein hydrolysate and Ascophyllum nodosum derived biostimulant. Agronomy 12(4):809. https://doi.org/10.3390/agronomy12040809
Islam M, Jahan K, Sen A, Urmi TA, Haque MM (2023). Exogenous application of calcium ameliorates salinity stress tolerance of tomato (Solanum lycopersicum L.) and enhances fruit quality. Antioxidants 12(3):558. https://doi.org/10.3390/antiox12030558
Kabir MSN, Ali M, Lee WH, Cho SI, Chung SO (2020). Physicochemical quality changes in tomatoes during delayed cooling and storage in a controlled chamber. Agriculture 10(6):196. https://doi.org/10.3390/agriculture10060196
Kundu S, Karak S, Hansda NN, Thapa U, Rahaman AO (2023). Assessing the effects of biostimulant on tomato growth, yield and quality in open field condition. International Journal of Environment and Climate Change 13(12):1177-1187. https://doi.org/10.9734/ijecc/2023/v13i123782
Kyriacou MC, Rouphael Y, Di Gioia F, Kyratzis A, Serio F, Renna M, De Pascale S, Santamaria P (2016). Micro-scale vegetable production and the rise of microgreens. Trends in Food Science & Technology 57:103-115. https://doi.org/10.1016/j.tifs.2016.09.005
Lakshmi S, Ravichandran V, Anandakumar S, Senthil A, Arul L, Radhamani S, Anupriya R (2023). Foliar application of Ascophyllum nodosum on improvement of photosynthesis, fruit setting percentage, yield and quality of tomato (Solanum lycopersicum L.). Journal of Applied & Natural Science 15(3):961-971. https://doi.org/10.31018/jans.v15i3.4725
MacCarthy P (2001). The principles of humic substances. Soil Science 166(11):738-751.
Malécange M, Sergheraert R, Teulat B, Mounier E, Lothier J, Sakr S (2023). Biostimulant properties of protein hydrolysates: Recent advances and future challenges. International Journal of Molecular Sciences 24(11):9714. https://doi.org/10.3390/ijms24119714
Malusà E, Pinzari F, Canfora L (2016). Efficacy of biofertilizers: challenges to improve crop production. In microbial inoculants in sustainable agricultural productivity. Functional Applications. New Delhi: Springer India 2:17-40. https://doi.org/10.1007/978-81-322-2644-4
Mohan A, Krishnan R, Arshinder K, Vandore J, Ramanathan U (2023). Management of postharvest losses and wastages in the indian tomato supply chain a temperature controlled storage perspective. Sustainability 15(2):1331. https://doi.org/10.3390/su15021331
Mouhamed BA, Kasnazany SAS (2024). Impact of harvesting stages and postharvest treatments on the quality and storability of tomato fruits (Solanum lycopersicum L.) cv. Sangaw. Coatings 14(9):1143. https://doi.org/10.3390/coatings14091143
Nardi S, Pizzeghello D, Muscolo A, Vianello A (2002). Physiological effects of humic substances on higher plants. Soil Biology and Biochemistry 34(11):1527-1536. https://doi.org/10.1016/S0038-0717(02)00174-8
Olivares FL, Aguiar NO, Rosa RCC, Canellas LP (2015). Substrate biofortification in combination with foliar sprays of plant growth promoting bacteria and humic substances boosts production of organic tomatoes. Scientia Horticulturae 183:100-108. https://doi.org/10.1016/j.scienta.2014.11.012
Onyegbula AF, Fashanu TA, Babatunde O, Yusuf KA, Adole EB (2023). Studies on postharvest loss assessment of tomato fruits using aloe vera gel as mitigating agent. Magna Scientia Advanced Research and Reviews 08(1):114-122. https://doi.org/10.30574/msarr.2023.8.1.0078
Patanè C, Pellegrino A, Saita A, Calcagno S, Cosentino SL, Scandurra A, Cafaro VA (2025). A study on the effect of biostimulant application on yield and quality of tomato under long lasting water stress conditions. Heliyon 11:e2301879. https://doi.org/10.1016/j.heliyon.2024.e41187
Rouphael Y, Colla G, Giordano M, El-Nakhel C, Kyriacou MC, De Pascale S (2017). Foliar applications of a legume derived protein hydrolysate elicit dose dependent increases of growth, leaf mineral composition, yield and fruit quality in two greenhouse tomato cultivars. Scientia Horticulturae 226:353-360. https://doi.org/10.1016/j.scienta.2017.09.007
Ruiz-Cisneros MF, Ornelas-Paz JDJ, Olivas-Orozco GI, Acosta-Muñiz CH, Sepúlveda-Ahumada DR, Pérez-Corral DA, Rios-Velasco C, Salas-Marina M, Fernández-Pavía SP (2018). Efecto de Trichoderma spp. y hongos fitopatógenos sobre el crecimiento vegetal y calidad del fruto de jitomate. Revista Mexicana de Fitopatología 36:444-456. https://doi.org/10.18781/r.mex.fit.1804-5
Schiavon M, Pizzeghello D, Muscolo A, Vaccoro S, Francioso O, Nardi S (2010). High molecular size humic substances enhance phenylpropanoid metabolism in maize (Zea mays L.). Journal of Chemical Ecology 36:662-669. https://doi.org/10.1007/s10886-010-9790-6
SIAP. Anuario estadístico de la producción agrícola (2023). Servicio de Información Agroalimentaria y Pesquera. https://www.gob.mx/siap
Sun C, Bei K, Liu Y, Pan Z (2022). Humic acid improves greenhouse tomato quality and bacterial richness in rhizosphere soil. ACS Omega 7:29823-29831. https://doi.org/10.1021/acsomega.2c02663
Tolisano C, Priolo D, Brienza M, Puglia D, Del Buono D (2024). Do lignin nanoparticles pave the way for a sustainable nanocircular economy? Biostimulant effect of nanoscaled lignin in tomato plants. Plants 13:1839. https://doi.org/10.3390/plants13131839
Tringovska I (2011). The effects of humic and bio-fertilizers on growth and yield of greenhouse tomatoes. Acta Horticulturae 960:443-449. https://doi.org/10.17660/ActaHortic.2012.960.62
Turan M, Ekinci M, Argin S, Brinza M, Yildirim E (2023). Drought stress amelioration in tomato (Solanum lycopersicum L.) seedlings by biostimulant as regenerative agent. Frontiers in Plant Science 14:1211210. https://doi.org/10.3389/fpls.2023.1211210
Vessey JK (2003). Plant growth promoting rhizobacteria as biofertilizers. Plant and Soil 255:571-586. https://doi.org/10.1023/A:1026037216893
Wankhade RS, Charjan YD, Nage S, Magare PN, Lawhale A (2025). Assessing the effect of bio stimulant on growth, yield and quality of tomato (Solanum lycopersicum L.). Journal of Advances in Biology & Biotechnology 28:850-855. https://doi.org/10.9734/jabb/2025/v28i62446
Wellala CKD, Sha MRA, Ekanayake EMMD, Welegama HMVT (2023). Quality comparison of vine-ripe and postharvest - ripened seven tomato varieties harvested at different stages of maturities. Tropical Agriculturist 171:32-50. https://doi.org/10.4038/ta.v171i4.49
Yan R, Li S, Cheng Y, Kebbeh M, Huan C, Zheng X (2022). Melatonin treatment maintains the quality of cherry tomato by regulating endogenous melatonin and ascorbate-glutathione cycle during room temperature. Journal of Food Biochemistry 46:e14285. https://doi.org/10.1111/jfbc.14285
Yildirim E (2007). Foliar and soil fertilization of humic acid affect productivity and quality of tomato. Acta Agriculturae Scandinavica Section B-Soil and Plant Science 57(2):182-186. https://doi.org/10.1080/09064710600813107
Zakriya M, Hussain A, Mahdi AA, Yasmeen F, Kausar T, Rehman A, Yaqub S, Fatima P, Noreen S, Kabir K, Nisar R, Gorsi FI, Fatima H, Korma SA (2023). Effect of different types of ethylene scavengers used in different combinations, on the post-harvest quality and phytochemicals retention of tomatoes (Solanum lycopersicum L.). Chemical and Biological Technologies in Agriculture 10(1):90. https://doi.org/10.1186/s40538-023-00465-w
Zuzunaga-Rosas J, González-Orenga S, Tofei AM, Boscaiu M, Moreno-Ramón H, Ibáñez-Asensio S, Vicente O (2022). Effect of a biostimulant based on polyphenols and glycine betaine on tomato plants’ responses to salt stress. Agronomy 12(9):2142. https://doi.org/10.3390/agronomy12092142
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Copyright (c) 2025 Mariana BARRERA-VILLANUEVA, Nora A. SALAS-SALAZAR, América CHÁVEZ-MARTÍNEZ, María A. FLORES-CÓRDOVA, Mayra C. SOTO-CABALLERO, María Janeth RODRÍGUEZ-ROQUE, Jorge E. DÁVILA-AVIÑA
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