Ethanol extract of Argyreia acuta leaves attenuates NSAID-induced gastric inflammation in mice via antioxidant and immunomodulatory mechanisms
DOI:
https://doi.org/10.55779/nsb17312687Keywords:
Argyreia acuta, NSAID-induced gastric injury, oxidative stress, immunomodulation, gastroprotectionAbstract
Indomethacin, a widely used NSAID, induces gastric injury via prostaglandin depletion, oxidative stress, and immune imbalance. This study evaluated the gastroprotective effects of Argyreia acuta leaf ethanol extract (EEAAL) in a murine model of indomethacin-induced gastritis. Mice were treated with EEAAL (100-300 mg/kg) or omeprazole following indomethacin administration (50 mg/kg). EEAAL significantly restored gastric pH, reduced acidity and stomach weight, and attenuated oxidative damage by lowering MDA, H2O2, and MPO levels while enhancing GSH, GPx, and GR activity. Immunological markers including WBC count, NBT reduction, phagocytic activity, and immunoglobulin levels, were also normalized. Notably, a satellite group maintained these improvements post-treatment, indicating prolonged efficacy. These findings demonstrate that EEAAL protects against NSAID-induced gastric injury through antioxidant and immunomodulatory mechanisms, supporting its potential as a plant-based therapeutic for gastric disorders.
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Aly MH, Said AK, Farghaly AM, Eldaly DA, Ahmed DS, Gomaa MH, … Wally ME (2024). Protective effect of astaxanthin on indomethacin-induced gastric ulcerations in mice. Naunyn-Schmiedeberg's Archives of Pharmacology 397(12):9897-9907. https://doi.org/10.1007/s00210-024-03206-4
Bi WP, Man HB, Man MQ (2014). Efficacy and safety of herbal medicines in treating gastric ulcer: A review. World Journal of Gastroenterology 20(45):17020-17028. https://doi.org/10.3748/wjg.v20.i45.17020
Binh TD, Han LN, Son DH, Hoan DT, Huong NTT, Thuong VA, Thuy NT (2022). Đặc điểm các loài có giá trị làm thuốc thuộc chi bạc thau (Argyreia Lour.) ở Việt Nam. Báo Cáo Khoa Học Về Nghiên Cứu và Giảng Dạy Sinh Học ở Việt Nam - Hội Nghị Khoa Học Quốc Gia Lần Thứ 4 22:183-190. https://doi.org/10.15625/vap.2020.00022 (in Vietnamese)
Chen W (2021). Evaluation of anti-inflammatory effect of different polar parts of Argyreia acuta Lour. Medicinal Plant 12(4):24-27.
Cherrada N, Chemsa AE, Gheraissa N, Laib I, Gueboudji Z, EL-Shazly M, … Messaoudi M (2024). Gastroprotective efficacy of North African medicinal plants: A review on their therapeutic potential for peptic ulcers. Food Science & Nutrition 12(11):8793-8824. https://doi.org/10.1002/fsn3.4536
Galani VJ, Patel BG, Patel NB (2010). Argyreia speciosa (Linn. f.) sweet: A comprehensive review. Pharmacognosy Reviews 4(8):172-178. https://doi.org/10.4103/0973-7847.70913
Han YM, Song MY, Lee DY, Lee SW, Ahn HR, Yoo J, … Kim EH (2023). Gastric mucosal protective effects of Cinnamomum cassia in a rat model of ethanol-induced gastric injury. Nutrients 16(1):55. https://doi.org/10.3390/nu16010055
Jenkins P, Cross C, Abdo T, Youness H, Keddissi J (2024). A review of current evidence for the use of steroids in the medical intensive care unit. Diagnostics 14(14):1565. https://doi.org/10.3390/diagnostics14141565
Joseph A, Mathew S, Skaria BP, Sheeja EC (2011). Medicinal uses and biological activities of Argyreia speciosa Sweet (Hawaiian Baby Woodrose) – An overview. Indian Journal of Natural Products and Resources 2(3):286-291.
Li Y, Zhu X, Mo L, Liu C, Li J, Li B, … Lu R (2021). Chemical constituents of the folk medicinal plant Argyreia acuta Lour. And their anti-inflammatory activity. Natural Product Communications 16(9):1-5. https://doi.org/10.1177/1934578X211044563
Loan LT, Oanh HV, Ha DT, Huyen CTT, Dung LV (2017). Nghiên cứu đặc điểm thực vật cây bạc thau (Argyreia acuta Lour.), họ Khoai lang (Convolvulaceae). Tạp Chí Dược Học 57(11):58-61. (in Vietnamese)
Maideen NMP (2023). Adverse effects associated with long-term use of proton pump inhibitors. Chonnam Medical Journal 59(2):115-127. https://doi.org/10.4068/cmj.2023.59.2.115
Muanrit P, Poomirat S, Sakpakdeejaroen I (2025). Validation of HPLC method for quantitative determination of zerumbone in the rhizome of Zingiber ottensii Valeton. Talanta Open 11:100405. https://doi.org/10.1016/j.talo.2025.100405
Nhung TTP, Quoc LPT (2023). Protective effects of black shallot extract against acetaminophen-induced nephrotoxicity in mice. Acta Biologica Szegediensis 67(2):177-185. https://doi.org/10.14232/abs.2023.2.177-185
Nhung TTP, Quoc LPT (2024a). The ethanol extraction of Gardenia stenophylla Merr fruit mitigates carbon tetrachloride-induced hepatic damage in mice through modulation of oxidative stress and immunosuppression. International Journal of Agricultural Technology 20(5):2015-2034.
Nhung TTP, Quoc LPT (2024b). Anti-oxidative stress and immunosuppressive effects of ethanol extract from sacha inchi leaves in mice with CFA-induced rheumatoid arthritis. Tropical Journal of Natural Product Research 8(9):8584-8592. https://doi.org/10.26538/tjnpr/v8i9.48
NRC - National Research Council (2022). The guide for the care and use of laboratory animals, Institute for Laboratory Animal Research (ILAR). Division of Earth and Life Studies (DELS). Guide for the Care and Use of Laboratory Animals 8th Ed., 248 pages.
Park JM, Hahm KB (2024). Dietary walnuts prevented indomethacin-induced gastric damage via AP-1 transcribed 15-PGDH, Nrf2-mediated HO-1, and n-3 PUFA-derived resolvin E1. International Journal of Molecular Sciences 25(13):7239. https://doi.org/10.3390/ijms25137239
Percie du Sert N, Hurst V, Ahluwalia A, Alam S, Avey MT, Baker M, … Würbel H (2020). The ARRIVE guidelines 2.0: Updated guidelines for reporting animal research. BMC Veterinary Research 16:242. https://doi.org/10.1186/s12917-020-02451-y
Pinthong W, Suanarunsawat T (2020). Tea seed oil alleviates metabolic derangement and oxidative stress in rats fed with high-fat and high fructose diet. Chiang Mai Journal of Science 19(4):665-683. https://doi.org/10.12982/CMUJNS.2020.0043
Riaz M, Khalid R, Afzal M, Anjum F, Fatima H, Zia S, … Aslam MA (2023). Phytobioactive compounds as therapeutic agents for human diseases: A review. Food Science & Nutrition 11:2500-2529. https://doi.org/10.1002/fsn3.3308
Rodríguez RR, Johnson JJ (2023). Terpenes: Modulating anti-inflammatory signaling in inflammatory bowel disease. Pharmacology & Therapeutics 248:108456. https://doi.org/10.1016/j.pharmthera.2023.108456
Roy A, Khan A, Ahmad I, Alghamdi S, Rajab BS, Babalghith AO, … Islam MR (2022). Flavonoids a bioactive compound from medicinal plants and its therapeutic applications. BioMed Research International 2022:5445291. https://doi.org/10.1155/2022/5445291
Rudrapal M, Khairnar SJ, Khan J, Dukhyil AB, Ansari MA, Alomary MN, … Devi R (2022). Dietary polyphenols and their role in oxidative stress-induced human diseases: Insights into protective effects, antioxidant potentials and mechanism(s) of action. Frontiers in Pharmacology 13:806470. https://doi.org/10.3389/fphar.2022.806470
Sakhri A, Sakhri FZ, Bensouici C (2024). Antioxidant, antibacterial, and immunomodulatory properties of Algerian Phoenix dactylifera. Natural and Life Sciences Communication 23(4):2024056. https://doi.org/10.12982/NLSC.2024.056
Shen L, Luo H, Fan L, Tian X, Tang A, Wu X, … Su Z (2023). Potential immunoregulatory mechanism of plant saponins: A review. Molecules 29(1):113. https://doi.org/10.3390/molecules29010113
Soydan M, Arabaci G, Utlu N, Halici MB, Senocak EA, Kiliçlioglu M (2025). Indomethacin-induced gastric ulcer in rats: Gastroprotectivity of Muscari neglectum in water. Pharmaceuticals 18(1):7. https://doi.org/10.3390/ph18010007
Tran PNT, Tran TTN (2021). Evaluation of acute and subchronic toxicity induced by the crude ethanol extract of Plukenetia volubilis Linneo leaves in Swiss albino mice. BioMed Research International 2021:6524658. https://doi.org/10.1155/2021/6524658
Wang X, Chen L, Chang L, He Y, He T, Wang R, … Zhao Y (2024). Mechanism of Wuzhuyu decoction on alcohol-induced gastric ulcers using integrated network analysis and metabolomics. Frontiers in Pharmacology 14:1308995. https://doi.org/10.3389/fphar.2023.1308995
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