Bioactive potential of Origanum elongatum extracts: Focus on phytochemical composition and antioxidant properties of different fractions

Oumaima HMIDOUCHE; Khadija BOUFTINI; Ibtissam SABIR; Mariama LASKY; Abderrahmane MRICHA; Halima RCHID; Rachid NMILA

doi:10.55779/nsb17312417

Authors

Oumaima HMIDOUCHE Chouaib Doukkali University, Faculty of Sciences, Laboratory of Biotechnology and Valorization of Plants Resources, El Jadida 24000 (MA) https://orcid.org/0009-0001-0115-8091
Khadija BOUFTINI Chouaib Doukkali University, Faculty of Sciences, Laboratory of Biotechnology and Valorization of Plants Resources, El Jadida 24000 (MA) https://orcid.org/0009-0000-0591-132X
Ibtissam SABIR Chouaib Doukkali University, Faculty of Sciences, Laboratory of Biotechnology and Valorization of Plants Resources, El Jadida 24000 (MA) https://orcid.org/0009-0004-9452-2594
Mariama LASKY Chouaib Doukkali University, Faculty of Sciences, Laboratory of Biotechnology and Valorization of Plants Resources, El Jadida 24000 (MA)
Abderrahmane MRICHA Chouaib Doukkali University, Faculty of Sciences, Laboratory of Biotechnology and Valorization of Plants Resources, El Jadida 24000 (MA)
Halima RCHID Chouaib Doukkali University, Faculty of Sciences, Laboratory of Biotechnology and Valorization of Plants Resources, El Jadida 24000; Chouaib Doukkali University, Polydisciplinary Faculty of Sidi Bennour, Sidi Bennour (MA) https://orcid.org/0000-0002-8017-8751
Rachid NMILA Chouaib Doukkali University, Faculty of Sciences, Laboratory of Biotechnology and Valorization of Plants Resources, El Jadida 24000; Chouaib Doukkali University, Higher School of Education and Training, El Jadida (MA) https://orcid.org/0000-0003-2529-7165

DOI:

https://doi.org/10.55779/nsb17312417

Keywords:

antioxidant activity, endemic plant, flavonoids, oxidative stress, polyphenols contents

Abstract

The variation of phenolic compounds and antioxidant activities of plant extracts is widely influenced by environmental factors, extraction methods and solvent polarity. Therefore, this study aimed to evaluate the phenolic composition and the antioxidant activities in vitro of Origanum elongatum aerial part, a perennial plant endemic to the Rif region of Morocco. It processed into crude, 80% methanolic extracts, and fractions obtained from it using hexane, dichloromethane, and ethyl acetate solvents. Total polyphenolic, flavonoid and proanthocyanidins contents were determined using the Folin-Ciocalteu, Andary, and vanillin methods. The antioxidant activity was assessed by four complementary methods DPPH, ABTS, FRAP and TAC. Preliminary phytochemical screening revealed the presence of saponins, essential oils, sterols, free Quinones, flavonoids, but the alkaloids were absent. Among the fractions, the hexane fraction (213.07 mg GAE.g^-1 of extract) and ethyl acetate fraction (193.93 mg GAE.g^-1 of extract) exhibited the high total phenolic content. Also, the hydromethanolic extract contained the highest flavonoids concentration (18.83 mg QE.g^-1). Notably, the ethyl acetate fraction consistently showed superior antioxidant activity across all assays, with an IC₅₀ of 0.17 ± 0.01 mg/mL for DPPH and 1.6 ± 0.01 μg/mL for ABTS. It also demonstrated the highest TAC 319.00 ± 0.05 mg EAA/g and FRAP values, confirming strong ferric reducing power. These results highlight the ethyl acetate fraction as a rich source of bioactive compounds with strong antioxidant properties, with a potential applications in pharmaceuticals, nutraceuticals, and food preservation. Further research is recommended to characterize its active constituents and investigate its biological efficacy in vivo.

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References

Abdelaali B, El Menyiy N, El Omari N, Benali T, Guaouguaou FE, Salhi N, Naceiri Mrabti H, Bouyahya A (2021). Phytochemistry, toxicology, and pharmacological properties of Origanum elongatum. Evidence‐Based Complementary and Alternative Medicine 2021:6658593. https://doi.org/10.1155/2021/6658593

Andary C. (1990). Documentation chimique et pharmaceutique pour l’AMM du MERALOPS comprimés. Laboratoire Allergan-Dulcis, Monaco, France.

Bakha M, El Mtili N, Machon N, Aboukhalid K, Amchra FZ, Khiraoui A, Gibernau M, Tomi F, Al Faiz C (2020). Intraspecific chemical variability of the essential oils of Moroccan endemic Origanum elongatum L. (Lamiaceae) from its whole natural habitats. Arabian Journal of Chemistry 13(1):3070-3081. https://doi.org/10.1016/j.arabjc.2018.08.015

Banso A, Adeyemo S (2006). Phytochemical screening and antimicrobial assessment of Abutilon mauritianum, Bacopa monnifera and Datura stramonium. Biokemistri 18(1):39-44. https://doi.org/10.4314/biokem.v18i1.56390

Belhacini N, Alem K, Boumendjel A, Messarah M (2023). Evaluation of Antioxidant and Dermato-protective activities of Origanum vulgare extracts. Research Journal of Pharmacy and Technology 16(12):5631-5636. https://doi.org/10.52711/0974-360X.2023.00910

Belmehdi O, El Harsal A, Benmoussi M, Laghmouchi Y, Senhaji NS, Abrini J (2018). Effect of light, temperature, salt stress and pH on seed germination of medicinal plant Origanum elongatum (Bonnet) Emb. & Maire. Biocatalysis and Agricultural Biotechnology 16:126-131. https://doi.org/10.1016/j.bcab.2018.07.032

Bendifallah L, Tchoulak Y, Djouabi M, Oukili M, Ghezraoui R (2015). Phytochemical study and antimicrobial activity of Origanum vulgare L. (Lamiaceae) in boumerdes mountainous region (Algeria). Journal of Medical and Bioengineering 4(6):471-474. https://doi.org/10.12720/jomb.4.6.471-474

Boukhira S, Bousta F, Moularat S, Abdellaoui A, Ouaritini ZB, Bousta D (2020). Evaluation of the preservative properties of Origanum elongatum essential oil in a topically applied formulation under a challenge test. Phytothérapie 18(2):92-98. https://doi.org/10.3166/phyto-2018-0067

Bruneton J. (2008). Pharmacognosy: Phytochemistry, medicinal plants. Editions Médicales.

Carović-Stanko K, Petek M, Grdiša M, Pintar J, Bedeković D, Herak Ćustić M, Satovic Z (2016). Medicinal plants of the family Lamiaceae as functional foods—A review. Czech Journal of Food Sciences 34(5):377-390. https://doi.org/10.17221/504/2015-cjfs

Dhull SB, Kaur P, Purewal SS (2016). Phytochemical analysis, phenolic compounds, condensed tannin content and antioxidant potential in Marwa (Origanum majorana) seed extracts. Resource-Efficient Technologies 2(4):168-174. https://doi.org/10.1016/j.reffit.2016.09.003

El Yousfi K, Greche H, Misbahi H, Ben Cheikh R (2020). Phyochemical evaluation and vasodilatory activity of O. elongatum, C. salviifolius and C. laurifolius. Moroccan Journal of Chemistry 8(1):226-234. https://doi.org/10.48317/IMIST.PRSM/morjchem-v8i1.19097

Evans WC (2009). Trease and Evans’ pharmacognosy (16th ed). Elsevier Health Sciences.

Hari A, Echchgadda G, Taarji N, Sahri N, Sobeh M, Ezrari S, Laasli SE, Lahlali R (2024). Chemical composition, antioxidant properties, and antifungal activity of wild Origanum elongatum extracts against Phytophthora infestans. Frontiers in Plant Science 15:1278538. https://doi.org/10.3389/fpls.2024.1278538

Heckmann M, Stadlbauer V, Drotarova I, Gramatte T, Feichtinger M, Arnaut V, Atzmüller, S, Schwarzinger B, Röhrl C, Blank-Landeshammer B, Weghuber J (2024). Identification of Oxidative-stress-reducing plant extracts from a novel extract library—comparative analysis of cell-free and cell-based in vitro assays to quantitate antioxidant activity. Antioxidants 13(3):297. https://doi.org/10.3390/antiox13030297

Kubola J, Siriamornpun S (2008). Phenolic contents and antioxidant activities of bitter gourd (Momordica charantia L.) leaf, stem and fruit fraction extracts in vitro. Food Chemistry 110(4):881-890. https://doi.org/10.1016/j.foodchem.2008.02.076

Nakamura Y, Tsuji S, Tonogai Y (2003). Analysis of proanthocyanidins in grape seed extracts, health foods and grape seed oils. Journal of Health Science 49(1):45-54. https://doi.org/10.1248/jhs.49.45

Oualili H, Nmila R, Chibi F, Lasky M, Mricha A, Rchid H (2019). Chemical composition and antioxidant activity of Origanum elongatum essential oil. Pharmacognosy Research 11(3):283-289. https://doi.org/10.4103/pr.pr_157_18

Oyaizu M (1986). Studies on products of browning reaction antioxidative activities of products of browning reaction prepared from glucosamine. The Japanese Journal of Nutrition and Dietetics 44(6):307-315.

Özcan MM, Erel Ö, Herken EE (2009). Antioxidant activity, phenolic content, and peroxide value of essential oil and extracts of some medicinal and aromatic plants used as condiments and herbal teas in Turkey. Journal of Medicinal Food 12(1):198-202. https://doi.org/10.1089/jmf.2008.0062

Prieto P, Pineda M, Aguilar M (1999). Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Analytical Biochemistry 269(2):337-341.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine 26(9-10):1231-1237.

Ribéreau-Gayon P, Gautheret R-J (1968). Les composés phénoliques des végétaux. Dunod, Paris, France.

Singleton VL, Orthofer R, Lamuela-Raventós RM (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology 299:152-178. https://doi.org/10.1016/S0076-6879(99)99017-1

Tagnaout I, Zerkani H, Amine S, Fadili K, Benhlima N, Bouzoubaa A, Zair T (2020). Phenolic composition and antioxidant potential of different solvent extracts of the endemic Origanum elongatum (Bonnet) Emberger & Maire. Mediterranean Journal of Chemistry 10(2):146-154. https://doi.org/10.13171/mjc10202002111248ittz

Tagnaout I, Zerkani H, Bencheikh N, Amalich S, Bouhrim M, Mothana RA, Alhuzani MR, Bouharroud R, Hano C, Zair T (2023). Chemical composition, antioxidants, antibacterial, and insecticidal activities of Origanum elongatum (Bonnet) Emberger & Maire aerial part essential oil from Morocco. Antibiotics 12(1):174. https://doi.org/10.3390/antibiotics12010174

Tumilaar SG, Hardianto A, Dohi H, Kurnia D (2024). A comprehensive review of free radicals, oxidative stress, and antioxidants: Overview, clinical applications, global perspectives, future directions, and mechanisms of antioxidant activity of flavonoid compounds. Journal of Chemistry 2024(1):5594386. https://doi.org/10.1155/2024/5594386