Anti-obesity activities of n-hexane fraction of Pterocarpus mildbraedii leaves on obese male rats

Sarah A. AKANDE; Uju D. ILIEMENE; Gideon A. GYEBI; Gabriel O. ANYANWU

doi:10.55779/nsb17212416

Authors

Sarah A. AKANDE Sheda Science and Technology Complex, Biotechnology Advanced Research Centre, Abuja (NG) https://orcid.org/0000-0001-7799-7994
Uju D. ILIEMENE Bingham University, Karu, Department of Biochemistry, Nasarawa State (NG) https://orcid.org/0000-0003-2368-1120
Gideon A. GYEBI Durban University of Technology, Faculty of Applied Sciences, Duban, Department of Biotechnology and Food Science (ZA) https://orcid.org/0000-0002-1945-1739
Gabriel O. ANYANWU Bingham University, Karu, Department of Biochemistry, Nasarawa State (NG) https://orcid.org/0000-0003-3110-2627

DOI:

https://doi.org/10.55779/nsb17212416

Keywords:

adipogenesis, high fat diet, lipolysis, obesity, Pterocarpus mildbraedii

Abstract

This study investigated the in vivo anti-obesity potentials of n-hexane fraction of Pterocarpus mildbraedii Harms in high fat diet induced obese rats. Gas chromatography mass spectrometry analysis showed eighteen peaks depicting the presence of seventeen bioactive compounds. Oral administration of n- hexane fraction of P. mildbraedii resulted in an appreciable loss (p < 0.05) in body and organ weight, significant improvement (p < 0.05) in blood lipid profile, characterized by decreased serum levels of triglycerides, total cholesterol, and low-density lipoprotein cholesterol, significantly lower (p < 0.05) glucose, insulin, and leptin levels, in contrast with adiponectin level. Moreover, treatment with n-hexane fraction of P. mildbraedii led to a significant (p < 0.05) downregulation of adipogenic genes and an upregulation of lipolytic genes via the AMPK signalling pathway. Additionally, histological examination showed the ameliorative effect of the fraction on liver and adipose tissues. Conclusively, results from this study propose P. mildbraedii as a viable option for the treatment and management of obesity, because it effectively reduced body weight, enhanced lipid profile, inhibit lipogenesis and promote lipolysis.

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