Isolation and characterization of bioplastic-degrading bacteria from nutmeg plantation land

Joseph PAGAYA; Syarifah Z.A. ALAYDRUS; Cecilia A. SEUMAHU; Melda YUNITA; Ritha TAHITU; Morgan OHIWAL

doi:10.55779/nsb17212363

Authors

Joseph PAGAYA Pattimura University, Faculty of Mathematics and Natural Sciences, Department of Biology, Jl. Ir. M. Putuhena, Poka, Ambon 97233, Indonesia (ID) https://orcid.org/0009-0009-7454-4231
Syarifah Z.A. ALAYDRUS Pattimura University, Faculty of Mathematics and Natural Sciences, Department of Biology, Jl. Ir. M. Putuhena, Poka, Ambon 97233, Indonesia (ID)
Cecilia A. SEUMAHU Pattimura University, Faculty of Mathematics and Natural Sciences, Department of Biology, Jl. Ir. M. Putuhena, Poka, Ambon 97233, Indonesia (ID)
Melda YUNITA Pattimura University, Faculty of Medicine, Department of Microbiology, Jl. Ir. M. Putuhena, Poka, Ambon 97233, Indonesia (ID) https://orcid.org/0000-0001-6668-8459
Ritha TAHITU Pattimura University, Faculty of Medicine, Department of Public Health, Jl. Ir. M. Putuhena, Poka, Ambon 97233 (ID)
Morgan OHIWAL Muhammadiyah University of Maluku, Faculty of Agriculture and Fisheries, Department of Agriculture, Wara, Ambon 91921 (ID) https://orcid.org/0000-0002-6092-8148

DOI:

https://doi.org/10.55779/nsb17212363

Keywords:

bioplastics, Bacillus, degrading bacteria, nutmeg plantation, Pseudomonas

Abstract

Excessive use of plastic in life can result in the accumulation of plastic waste that endangers the environment. Plastics are made from fossil fuels, coal, petroleum, and natural gas, which make plastic properties difficult to degrade. The application of bioplastics can overcome this problem because it comes from organic materials such as starch, cellulose, and lignin that can be decomposed. Bioplastics have the same functional quality as conventional plastics and are environmentally friendly because they can be degraded with the help of soil bacteria without producing toxic compounds. This study aimed to detect and determine the biodegradation potential of bioplastics by bacterial isolates obtained from the nutmeg plantation soil of West Seram Regency, Maluku, Indonesia. This study used a completely randomized design (CRD). Nutmeg plantation soil samples were taken from 5 different points, which were given 3 treatments on soil weight, namely P1, P2, and P3, as well as control using a mixture of 5 points of soil samples and adding Pseudomonas putida. There were 6 isolates characterized as having similarities with the genus Pseudomonas and Bacillus from nutmeg plantation soil samples. The percentage of biodegradation of genus Pseudomonas from isolates S1I2.1 was 40.02%, S2I2.2 of 48.08%, S2I1.1 of 65.41%. While genus Bacillus from isolate S1I2.1 showed a biodegradation value of 63.83%, followed by isolates S2I3.1 of 58.37%, and S3I2.1 of 55.48%. In conclusion, bacteria isolated from nutmeg plantation soil are known to have the potential to degrade bioplastics as seen from their ability to reduce plastic contained in investigated samples.

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