Probiotic potential of Enterococcus strains with multiple enterocin-encoding genes


  • Zahia BENMOUNA University of Sciences and Technology of Oran Mohamed Boudiaf, USTOMB, Faculty of Natural and Life Sciences, Department of Living and Environment, PO Box 1505, El Mnaouar, Oran 31000; University of Science and Technology Houari Boumediene, USTHB, Faculty of Biological Sciences, Laboratory of Research in Arid Areas, PO Box 32, El Alia, Bab Ezzouar, Algiers 16111 (DZ)
  • Eva VALDIVIA Universidad de Granada, Departamento de Microbiología, Avda. Fuentenueva, s/n, Granada 18071 (ES)
  • Manuel MONTALBÁN-LÓPEZ Universidad de Granada, Departamento de Microbiología, Avda. Fuentenueva, s/n, Granada 18071 (ES)
  • Fatiha DALACHE University of Mostaganem Abdelhamid Ibn Badis, Faculty of Natural and Life Sciences, Department of Biology, PO Box 188, Mostaganem 27000; University of Oran1, Laboratory of Microorganisms Biology and Biotechnology, PO Box 1524, El M’Naouer, Oran 31000 (DZ)
  • Halima ZADI-KARAM University of Oran1, Laboratory of Micro-organisms Biology and Biotechnology, PO Box 1524, El M’Naouer, Oran 31000 (DZ)
  • Nour-Eddine KARAM University of Oran1, Laboratory of Micro-organisms Biology and Biotechnology, PO Box 1524, El M’Naouer, Oran 31000 (DZ)
  • Manuel MARTÍNEZ-BUENO Universidad de Granada, Departamento de Microbiología, Avda. Fuentenueva, s/n, Granada 18071 (ES)



enterocins, Enterococcus, lactic acid bacteria, probiotics, virulence factors


In this work, we sought to identify and characterize three strains of Enterococcus isolated from camel milk and fermented wheat, based on the capacity to produce bacteriocins and the probiotic potential. Polymerase chain reaction analyses were used to identify bacterial isolates and structural genes of bacteriocins, and also to detect potential enterococcal virulence genes (cylA, esp, gelE, efaAfs, hyl, ace, asa1, vanA, and vanB). The antimicrobial activity of the strains was investigated in solid media by the agar spot method against several pathogenic bacteria. The probiotic potential of the strains was also analysed using low pH (pH 3.0), bile salt resistance, DNAase, and antibiotic susceptibility assays. The strains were identified using rRNA16S sequencing gene, showing their belonging to E. faecium species. Based on PCR results, E. faecium CM9 and CM18 strains included in the genome the structural gene of enterocin A, enterocin B, and enterocin P, while E. faecium H3 possessed enterocin MR10A/B structural gene. The sequence analysis revealed that the H3 strain included in the genome the structural gene of enterocin L50A/B. All the pathogenic bacteria (Staphylococcus aureus, Salmonella enterica, Listeria monocytogenes, E. coli, and Pseudomonas aeruginosa) were inhibited by the three strains E. faecium CM9, CM18, and H3. In the well diffusion test, the supernatants of the three strains exhibited inhibitory activity against Listeria monocytogenes CECT 4032. The strains showed high tolerance to low pH and bile salts, did not possess DNase, were susceptible to the majority of antibiotics assayed, and did not possess any of the virulence genes analyzed in this study. A promising candidate strains were identified as potential probiotics with anti-bacterial action against pathogenic bacteria.


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How to Cite

BENMOUNA, Z., VALDIVIA, E., MONTALBÁN-LÓPEZ, M., DALACHE, F., ZADI-KARAM, H., KARAM, N.-E., & MARTÍNEZ-BUENO, M. (2024). Probiotic potential of Enterococcus strains with multiple enterocin-encoding genes. Notulae Scientia Biologicae, 16(1), 11792.



Research articles
DOI: 10.55779/nsb16111792