Antifungal activity against Candida albicans biofilm of Coffea robusta monofloral honey from Vietnam

Authors

  • Quoc-Dat DANG University of Science, Faculty of Biology and Biotechnology, Department of Molecular and Environmental Biotechnology, Laboratory of Biosensors, 227 Nguyen Van Cu, Ho Chi Minh City 700000; University of Science, Laboratory of Molecular Biotechnology, 227 Nguyen Van Cu, Ho Chi Minh City 700000; Vietnam National University, Quarter 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam 700000 (VN)
  • Thuy-Trinh T. NGUYEN University of Science, Faculty of Biology and Biotechnology, Department of Molecular and Environmental Biotechnology, Laboratory of Biosensors, 227 Nguyen Van Cu, Ho Chi Minh City 700000; University of Science, Laboratory of Molecular Biotechnology, 227 Nguyen Van Cu, Ho Chi Minh City 700000; Buon Ma Thuot Medical University, Faculty of Medicine, Department of Biochemistry, 298 Ha Huy Tap, Buon Ma Thuot, Dak Lak Province 630000 (VN)
  • Chau D. BUI University of Science, Faculty of Biology and Biotechnology, Department of Molecular and Environmental Biotechnology, Laboratory of Biosensors, 227 Nguyen Van Cu, Ho Chi Minh City 700000; University of Science, Laboratory of Molecular Biotechnology, 227 Nguyen Van Cu, Ho Chi Minh City 700000; Vietnam National University, Quarter 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam 700000 (VN)
  • Hieu TRAN-VAN University of Science, Faculty of Biology and Biotechnology, Department of Molecular and Environmental Biotechnology, Laboratory of Biosensors, 227 Nguyen Van Cu, Ho Chi Minh City 700000; University of Science, Laboratory of Molecular Biotechnology, 227 Nguyen Van Cu, Ho Chi Minh City 700000; Vietnam National University, Quarter 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam 700000 (VN)

DOI:

https://doi.org/10.55779/nsb16111600

Keywords:

antifungal activity, biofilm, Candida albicans, honey, minimum inhibitory concentration (MIC), transcription level

Abstract

Coffee (Coffea robusta) flower honey is one of the highest-yielding kinds of honey in Vietnam. Nevertheless, there is little research on the antifungal activity associated with this type of honey. Therefore, this study was conducted to investigate the antifungal activity against C. albicans of Vietnamese coffee monofloral honey and its potential effects on biofilm formation. This study was conducted using the broth microdilution method. The results showed that coffee flower honey was resistant to Candida albicans strain with minimum inhibitory concentration (MIC) and with minimum fungicidal concentration (MFC) values of 50% and 70% (w/v), respectively. No change in the antifungal activity of honey and the antifungal agent (Amphotericin B) against the C. albicans strain occurred after five consecutive exposures. When using SEM, honey at MIC (w/v) strongly affected the two stages of C. albicans biofilm formation. The surface structure of the biofilm, deformed, distorted, and atrophied phenotypes impacted by honey were also observed. Expression of transcriptional-level genes involved in biofilm formation, quorum-sensing, and stress survival was analyzed by RT-qPCR in honey-treated and untreated biofilms. The present study showed the antifungal activity of Vietnamese coffee flower honey against C. albicans.

Metrics

Metrics Loading ...

References

Anand S, Deighton M, Livanos G, Pang ECK, Mantri N (2019). Agastache honey has superior antifungal activity in comparison with important commercial honeys. Scientific Reports 9(1):18197. https://doi.org/10.1038/s41598-019-54679-w

Ansari MJ, Al-Ghamdi A, Usmani S, Al-Waili NS, Sharma D, Nuru A, Al-Attal Y (2013). Effect of jujube honey on Candida albicans growth and biofilm formation. Archives of Medical Research 44(5):352-360. https://doi.org/10.1016/j.arcmed.2013.06.003

Bang LM, Buntting C, Molan P (2003). The effect of dilution on the rate of hydrogen peroxide production in honey and its implications for wound healing. Journal of Alternative and Complementary Medicine 9(2):267-273. https://doi.org/10.1089/10755530360623383

Biswas S, Van Dijck P, Datta A (2007). Environmental sensing and signal transduction pathways regulating morphopathogenic determinants of Candida albicans. Microbiology and Molecular Biology Reviews 71(2):348-376. https://doi.org/10.1128/MMBR.00009-06

Blair SE, Cokcetin NN, Harry EJ, Carter DA (2009). The unusual antibacterial activity of medical-grade Leptospermum honey: antibacterial spectrum, resistance and transcriptome analysis. European Journal of Clinical Microbiology & Infectious Diseases 28(10):1199-1208. https://doi.org/10.1007/s10096-009-0763-z

Chen C, Campbell LT, Blair SE, Carter DA (2012). The effect of standard heat and filtration processing procedures on antimicrobial activity and hydrogen peroxide levels in honey. Frontiers in Microbiology 3:265. https://doi.org/10.3389/fmicb.2012.00265

Costa-de-Oliveira S, Rodrigues AG (2020). Candida albicans antifungal resistance and tolerance in bloodstream infections: the triad yeast-host-antifungal. Microorganisms 8(2). https://doi.org/10.3390/microorganisms8020154

da Silva PM, Gauche C, Gonzaga LV, Costa AC, Fett R (2016). Honey: Chemical composition, stability and authenticity. Food Chemistry 196:309-323. https://doi.org/10.1016/j.foodchem.2015.09.051

Dominic RM, Shenoy S, Baliga S (2007). Candida biofilms in medical devices: evolving trends. Kathmandu University Medical Journal 5(19):431-436.

Fernandes L, Ribeiro H, Oliveira A, Sanches Silva A, Freitas A, Henriques M, Rodrigues ME (2021). Portuguese honeys as antimicrobial agents against Candida species. Journal of Traditional and Complementary Medicine 11(2):130-136. https://doi.org/10.1016/j.jtcme.2020.02.007

Hermanns R, Mateescu C, Thrasyvoulou A, Tananaki C, Wagener FADTG and Cremers NAJ (2019). Defining the standards for medical grade honey. Journal of Apicultural Research 59(2):125-135. https://doi.org/10.1080/00218839.2019.1693713

Hornby JM, Jensen EC, Lisec AD, Tasto JJ, Jahnke B, Shoemaker R, … Nickerson KW (2001). Quorum sensing in the dimorphic fungus Candida albicans is mediated by farnesol. Applied and Environmental Microbiology 67(7):2982-2992. https://doi.org/10.1128/AEM.67.7.2982-2992.2001

Kamal MM, Rashid MHU, Mondal SC, El Taj HF, Jung C (2019). Physicochemical and microbiological characteristics of honey obtained through sugar feeding of bees. Journal of Food Science and Technology 56(4):2267-2277. https://doi.org/10.1007/s13197-019-03714-9

Kebaara BW, Langford ML, Navarathna DH, Dumitru R, Nickerson KW, Atkin AL (2008). Candida albicans Tup1 is involved in farnesol-mediated inhibition of filamentous-growth induction. Eukaryote Cell 7(6):980-987. https://doi.org/10.1128/EC.00357-07

Koc AN, Silici S, Ercal BD, Kasap F, Hormet-Oz HT, Mavus-Buldu H (2009). Antifungal activity of Turkish honey against Candida spp. and Trichosporon spp: an in vitro evaluation. Medical Mycology 47(7):707-712. https://doi.org/10.3109/13693780802572554

Leach MD, Farrer RA, Tan K, Miao Z, Walker LA, Cuomo CA, … Cowen LE (2016). Hsf1 and Hsp90 orchestrate temperature-dependent global transcriptional remodelling and chromatin architecture in Candida albicans. Nature Communications 7:11704. https://doi.org/10.1038/ncomms11704

Montenegro G, Velásquez P, Viteri R, Giordano A (2021). Changes in the antibacterial capacity of Ulmo honey in relation to the contribution of Eucryphia cordifolia pollen. Journal of Food and Nutrition Research 60(3):279-283.

Morici P, Fais R, Rizzato C, Tavanti A, Lupetti A (2016). Inhibition of Candida albicans biofilm formation by the synthetic lactoferricin derived peptide hLF1-11. PLoS One 11(11):e0167470. https://doi.org/10.1371/journal.pone.0167470

Nair R, Khandelwal NK, Shariq M, Redhu AK, Gaur NA, Shaikh S, Prasad R (2018). Identification of genome-wide binding sites of heat shock factor 1, Hsf1, under basal conditions in the human pathogenic yeast, Candida albicans. AMB Express 8(1):116. https://doi.org/10.1186/s13568-018-0647-7

Nobile CJ, Johnson AD (2015). Candida albicans biofilms and human disease. Annual Review of Microbiology 69(71-92. https://doi.org/10.1146/annurev-micro-091014-104330

Perea S, Patterson TF (2002). Antifungal resistance in pathogenic fungi. Clinical Infectious Disease 35(9):1073-1080. https://doi.org/10.1086/344058

Ramage G, VandeWalle K, Lopez-Ribot JL, Wickes BL (2002). The filamentation pathway controlled by the Efg1 regulator protein is required for normal biofilm formation and development in Candida albicans. FEMS Microbiology Letter 214(1):95-100. https://doi.org/10.1111/j.1574-6968.2002.tb11330.x

Rodrigues CF, Goncalves B, Rodrigues ME, Silva S, Azeredo J, Henriques M (2017). The effectiveness of voriconazole in therapy of Candida glabrata's biofilms oral infections and its influence on the matrix composition and gene expression. Mycopathologia 182(7-8):653-664. https://doi.org/10.1007/s11046-017-0135-7

Santos-Buelga C, González-Paramás AM (2017). Chemical Composition of Honey. In: Alvarez-Suarez JM (Ed). Bee Products - Chemical and Biological Properties. Springer Cham., pp 43-82. https://doi.org/10.1007/978-3-319-59689-1_3

Sun L, Liao K, Wang D (2015). Effects of magnolol and honokiol on adhesion, yeast-hyphal transition, and formation of biofilm by Candida albicans. PLoS One 10(2):e0117695. https://doi.org/10.1371/journal.pone.0117695

Tomičić Z, Tomičić R, Možina SS, Bucar F, Turek I, Raspor P (2022). Antifungal and anti-adhesion activity of plant extracts and essential oils against Candida spp. and Pichia spp. Journal of Food and Nutrition Research 61(1):61-68.

Vica ML, Glevitzky M, Dumitrel GA, Bostan R, Matei HV, Kartalska Y, Popa M (2022). Qualitative characterization and antifungal activity of romanian honey and propolis. Antibiotics (Basel) 11(11). https://doi.org/10.3390/antibiotics11111552

Zabouri Y, Cheriguene A, Chougrani F, Merzouk Y, Marchetta A, Urzi C, Leo FD (2021). Antifungal activity of lactic acid bacteria against phytopathogenic Alternaria alternata species and their molecular characterization. Journal of Food and Nutrition Research 60(1):18-28.

Zhao LX, Li DD, Hu DD, Hu GH, Yan L, Wang Y, Jiang YY (2013). Effect of tetrandrine against Candida albicans biofilms. PLoS One 8(11):e79671. https://doi.org/10.1371/journal.pone.0079671

Downloads

Published

2024-03-29

How to Cite

DANG, Q.-D., NGUYEN, T.-T. T., BUI, C. D., & TRAN-VAN, H. (2024). Antifungal activity against Candida albicans biofilm of Coffea robusta monofloral honey from Vietnam. Notulae Scientia Biologicae, 16(1), 11600. https://doi.org/10.55779/nsb16111600

Issue

Section

Research articles
CITATION
DOI: 10.55779/nsb16111600